Journal: Materials Today Bio

Article Title: Sequential delivery of sinigrin and dabigatran by an in situ self-stabilizing dynamic hydrogel attenuates intervertebral disc degeneration

doi: 10.1016/j.mtbio.2026.102827

Figure Lengend Snippet: Metabolic regulation of nucleus pulposus vells by SIN and DAB through MAPK and NF-κB pathways. (A) Volcano plot analysis of DE genes (TNF-α vs. TNF-α+SIN). (B) KEGG enrichment analysis of DE genes (TNF-α vs. TNF-α+SIN). (C) GSEA indicated MAPK signalling pathway enrichment. (D) Heatmap of ten selected differentially expressed genes (TNF-α vs. TNF-α+SIN). (E) Western blot analysis of key proteins in the MAPK signalling pathway (p-ERK, p-JNK, p-p38, ERK, JNK, and p38) in the different treatment groups. (F) Quantitative analysis of the Western blot data for MAPK signalling pathway proteins. (G–H) Western blot and quantitative analysis of p-ERK, p-JNK, p-p38, ERK, JNK, and p38 treated with or without TNF-α in the absence or presence of SIN (10 μM) (PCDNA 3.1 vs. TNFR1-OE).(I–L) Integrated analysis of TNF-α vs. TNF-α+DAB DEGs: Volcano plot of DE genes (significance/fold change), KEGG pathway enrichment of DE genes, NF-κB signalling enrichment in GSEA, and heatmap of ten selected differentially expressed genes. (M) Western blot analysis of key proteins in the NF-κB signalling pathway (p-IκBα, p-p65, IκBα, and p65) in the different treatment groups. (N) Quantitative analysis of the Western blot data for the NF-κB signalling pathway proteins. (O–P) Western blot and quantitative analysis of p-p65 and p65 (PCDNA 3.1 vs. TNFR1-OE). (Q) Matched diagram of b and y ions. (R) Western blot analysis of RELA in the different treatment groups. (S) Western blot analysis confirmed the stabilization of p65 by DAB. (T) The affinity between DAB and the p65 target protein was estimated after incubation at different temperatures via Western blot analysis. (n = 3; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001).

Article Snippet: The membranes were then incubated overnight with anti-GAPDH (1:50 000, AC054, abclone, China), anti-Col2α1(1:500, A19308, abclone, China), anti-Acan (1:1000, A8536, abclone, China), anti-SOX9 (1:1000, A19710, abclone, China), anti-MMP13 (1:1000, A1606, abclone, China), anti-ADAMTS4 (1:500, A2525, abclone, China), anti-iNOS (1:1000, 18985-1-AP, proteintech, China), anti-COX2 (1:1000, 12375-1-AP, proteintech, China), anti-ERK (1:2000, 11257-1-AP, proteintech, China), anti-Phospho-ERK (1:1000, 28733-1-AP, proteintech, China), anti-JNK (1:500, 51153-1-AP, proteintech, China), anti-Phospho-JNK (1:1000, 80024-1-RR, proteintech, China), anti-p38 (1:1000, A4771, abclone, China), anti-Phospho-p38 (1:500, AP1508, abclone, China), anti-p65 (1:5000, A19653, abclone, China), anti-Phospho-p65 (1:500, AP0475, abclone, China), anti-IκBα (1:500, A1187, abclone, China), anti-Phospho-IκBα (1:500, AP0420, abclone, China), anti-AMPK (1:5000, A28094, abclone, China), anti-Phospho-AMPK (1:500, AP0432, abclone, China), The membrane was incubated with HRP-conjugated Goat anti-rabbit IgG (H + L) (1:50 000, AS014, rabbit monoclonal, Abclone, China) and immunoreactive proteins were detected using enhanced chemiluminescence.

Techniques: Western Blot, Incubation